http://www.plosone.org/article/info%3Ad ... ne.0053087
Frondoside A Suppressive Effects on Lung Cancer Survival, Tumor Growth, Angiogenesis, Invasion, and Metastasis
Abstract
A major challenge for oncologists and pharmacologists is to develop less toxic drugs that will improve the survival of lung cancer patients. Frondoside A is a triterpenoid glycoside isolated from the sea cucumber, Cucumaria frondosa and was shown to be a highly safe compound. We investigated the impact of Frondoside A on survival, migration and invasion in vitro, and on tumor growth, metastasis and angiogenesis in vivo alone and in combination with cisplatin. Frondoside A caused concentration-dependent reduction in viability of LNM35, A549, NCI-H460-Luc2, MDA-MB-435, MCF-7, and HepG2 over 24 hours through a caspase 3/7-dependent cell death pathway. The IC50 concentrations (producing half-maximal inhibition) at 24 h were between 1.7 and 2.5 µM of Frondoside A. In addition, Frondoside A induced a time- and concentration-dependent inhibition of cell migration, invasion and angiogenesis in vitro. Frondoside A (0.01 and 1 mg/kg/day i.p. for 25 days) significantly decreased the growth, the angiogenesis and lymph node metastasis of LNM35 tumor xenografts in athymic mice, without obvious toxic side-effects. Frondoside A (0.1–0.5 µM) also significantly prevented basal and bFGF induced angiogenesis in the CAM angiogenesis assay. Moreover, Frondoside A enhanced the inhibition of lung tumor growth induced by the chemotherapeutic agent cisplatin. These findings identify Frondoside A as a promising novel therapeutic agent for lung cancer.
Figure 5. The anti-angiogenic activity of Frondoside A in the Chorioallantoic membrane (CAM) assay in vivo:
CAM was treated with control (serum-free medium), 100 nM Frondoside A, 500 nM Frondoside A, 2 µg/mL bFGF, 2 µg/mL bFGF+500 nM Frondoside A and the vascularization of test discs was photographed.
doi:10.1371/journal.pone.0053087.g005
Znalazłam sklep, ale niestety ceny są zawrotne: http://www.kerafast.com/p-1193-frondoside-a.aspxStatystyki: Napisane przez dziwozona — Pt lis 08, 2013 4:55 pm
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